TruDiagnostic TruAge Review 2025: The Top Test to Quantify Your Biological Age & Longevity?

How old are you?

If you’re like most people, you instinctively thought of your calendar age (birth day and year).

But that’s not really what matters. We all know someone that’s 50 and looks 30 and vice versa.

BioAge depicts your cellular and molecular age. In other words, how old you really are.

Biological age testing reveals your true age. I’ve used all the top tools, and today I’m sharing my favorite.

Welcome to the TruDiagnostic TruAge review. In this post, I’ll share my personal experience, results, and the science to help you decide if it’s worth including in your longevity protocol.

What’s a Biological Age Test & Why Does it Matter?

A biological age test assesses how your body is aging internally at a cellular and molecular level.

Understanding your biological age allows you to:

• Get a snapshot of your current health status beyond traditional metrics
• Identify areas where you can slow the aging process
• Track the impact of interventions like diet, exercise, or supplements
• Make science-based decisions about your wellness and future

There are different types of biological age tests but the best one is epigenetic methylation.

DNA methylation measures chemical tags (methyl groups) added to DNA. Methylation silences the expression of genes without directly modifying them genes. Like an ‘off’ switch. These patterns change predictably with age, providing an accurate biological age estimate.

That’s why epigenetic methylation tests, like TruAge, are considered the most useful and accurate in biological age assessment.

The usefulness is no longer merely theoretical.

In a 2023 study of 2,950 ICU patients (whose biological ages were assessed upon admission), researchers stated:

“Biological age is increasingly recognized as being more accurate than chronological age in determining chronic health outcomes (Intern Emerg Med). ”

They found that:

• Biological age predicted mortality nearly 2X as well as chronological age
• Being biologically older than one’s chronological age was more common among those with comorbidities
• Patients whose biological age exceeded their actual age had almost 2X the risk of death
• The greater this gap between biological and chronological age, the higher the risk [of death]; the risk doesn’t plateau at all, even when the difference was 20 years (Intern Emerg Med)

Essentially, the higher your biological age, the greater your risk of dying from any cause.

What is TruAge & How Does it Work?

TruAge by TruDiagnostic is the best at-home epigenetic age test. TruDiagnostic is known as the first company to offer an accurate commercial biological age test.

TruAge analyzes over 900,000 DNA markers to estimate your biological age, showcasing the impact of your lifestyle (and supplement and drug regimen) on the expression of your genes.

Your TruAge reports will include your:

• Aging speed
• Organ health & age
• Disease risk
• And beyond

If you’re like me, you’re probably wondering about the accuracy of these tests.

TruDiagnostic’s algorithms are validated through 30+ ongoing clinical trials and partnerships with Harvard, Yale, and other leading universities. They use next generation algorithms to ensure accurate (and reproducible!) results.

The latest generation of biological aging clocks

The term “clocks” refers to sophisticated statistical models or algorithms that analyze DNA methylation data.

The quality of the clock is key to how accurately it predicts biological age and health outcomes. This is crucial because it determines how reliable the test results will be.

TruDiagnostic uses 3 clocks. These 2nd- and 3rd-generation clocks incorporate multi-layered biological data beyond just DNA methylation, improving accuracy and clinical relevance:

• DunedinPACE (Duke partnership)
• OMICm Age (Harvard partnership)
• SYMPHONYAge (Yale partnership)

DunedinPACE algorithm (Duke partnership)

TruDiagnostic’s TruAge was originally built on the open source DunedinPACE algorithm—a gold standard for estimating current aging speed rather than cumulative age.

When I evaluated the best epigenetic biological age testing kits, TruDiagnostic’s decision to use the respected open source DunedinPACE algorithm played a major role.

A test is only as good as its accuracy (and repeatability), and with an open source algorithm, you’re not hoping the team made the right choice

This algorithm has exceptional test-retest reliability (ICC = 0.96) and far superior predictive accuracy compared to traditional aging measures (eLife).

The sophistication and accuracy of these clocks make TruAge the most scientifically advanced and accurate test available today.

OMICm Age (Harvard partnership)

This is the first multiomic clock, meaning it combines DNA methylation with protein (proteomic), metabolites (metabolomic), genes (genomics), transcriptomics, and clinical data.

Developed using over 50 years of biobank samples, it predicts mortality with about 90% accuracy over ten years. Significantly outperforming chronological age predictions at ~75% accuracy (bioRxiv).

OMICm Age reports include data on:

• Biological age
• Fit Age
• Specific biomarkers

Besides determining biological age, it provides insights into the molecular reasons causing accelerated aging.

SYMPHONYAge (Yale partnership)

Developed with Yale University, this clock provides organ-specific aging analysis. SYMPHONYAge is built on both DNA methylation and proteomic markers.

SYMPHONYAge assesses 11 key organ systems:

• Brain
• Blood
• Lungs
• Heart
• Liver
• Hormone
• Immune system
• Inflammation
• Kidneys
• Metabolic
• Musculoskeletal

This assessment gives targeted insights into how different parts of your body are aging which likely differs from your overall biological age.

Nick’s TruAge Experience & Results: Testing 5 Days Post-Dry Fast

I took my first TruAge test back in 2022, and re-tested for the first time in 2025.

My TruAge sample collection kit that arrived included all the materials I needed to collect a blood sample at home:

• 1 instruction guide
• 1 mini blood spot card for collecting blood
• 2 fingertip lancets (small devices to prick the finger)
• 1 dry gauze bandage
• 1 sanitizing wet wipe
• 1 adhesive bandage
• 1 biohazard bag
• 1 prepaid envelope to return the sample to the lab

Following the instructions, I registered my TruAge kit and collected my blood sample. It involved pricking my finger with the included lancet (which was painless) and only required about 6 or 7 drops of blood.

Then, I filled out a short online questionnaire.

The entire process took about 15 minutes.

I sealed my sample in the biohazard bag, placed it into the prepaid envelope, and sent it in.

Due to some processing complications, my results took a full 2 months to come back. Usually, they’re faster. But TruDiagnostic more than made up for the delay.

While I waited, I confirmed with co-founder Ryan Smith that they do not use my chronological age (that I provided in the questionnaire). TruDiagnostic does not use calendar age in biological age calculation.

Some companies do, and it dramatically reduces the utility of the test results.

My results weren’t quite what I hoped. My 0.7 pace of aging is quite low, but my 5.2-year lower biological age isn’t as good as my previous test.

This means:

• My biological age is significantly younger than my chronological age
• My pace of aging ranked among the slowest recorded
• Several organ systems are performing well below my real age

The fasting context

I collected my blood sample for the test 5 days after a 2-day dry fast, which certainly influenced my results.

Initially, I had planned to complete a 5 day dry fast, but decided to cut it short.

Fasting is well-supported in the literature as a driver of beneficial methylation changes, especially in pathways related to metabolism and longevity:

• Fasting induces changes in DNA methylation, particularly in genes related to metabolism and aging (Clin Epigenetics)
• Caloric restriction delays methylation drift and can make blood methylation age appear 7 years younger (Nature Commun)
• Short-term fasting alters gene expression and methylation patterns within hours to days (J Mol Med)

In hindsight, I would’ve waited at least a week after the fasting, perhaps two weeks to capture the full effects.

As you’ll see soon in my next review, fasting appears to have altered some of my epigenetic biomarker proxies even more.

My TruAge results explained

Now, I’ll share my results, what they mean, and what I plan to do about them.

After all, what good are these tests if they’re not actionable?

OMICm biological age

According to the OMICm clock, my biological age is 25.2.

That means I’m 5.2 years younger than my actual age, placing me in the top youngest 30% of people my calendar age and sex.

The OMICm results also reported the most actionable epigenetic biomarkers I have. As you may see below, TruAge also gives recommendations for lifestyle changes/adjustments:

Of course, the results not just include these top-impact biomarkers but also other biomarkers that will help inform even small changes in lifestyle.

Little habits and practices you didn’t think would affect your health would suddenly become highlighted.

SYMPHONYAge

SYMPHONYAge provides a granular analysis, offering separate “ages” for 11 organ systems. This table shows some of my results.

The ability to see which systems are lagging helps inform future health optimization strategies.

DunedinPACE of Aging

For every chronological year, I accumulate only 0.7 biological years. At the time of writing, this would rank me in the top 15 on the Rejuvenation Olympics leaderboard (assuming I retested twice at the same rate).

OMICm Fit Age

Informs how old you are according to your physical fitness and functionality.

My results:

• Fit Age: 28.2 (2.2 years younger than chronological age)
• Grip Strength Percentile: 47.3%
• Gait Speed Percentile: 59.6%
• VO2 Max Percentile: 44.5%
• FEV1 (Lung Function) Percentile: 69.1%

These reinforce that my cardiorespiratory and musculoskeletal systems are notably resilient, key for healthy longevity.

Interpreting my TruAge results in context

TruAge’s multi-layered tests delivered actionable insights and tailored epigenetic recommendations.

TruAge report indicates opportunities to improve based on:

• Inflammation
• Gut health
• Blood sugar

I’ve already implemented some changes.

The effects of my recent dry fast, which is known to influence each of these, likely contributed to the outcomes.

For my DunedinPACE score, I’m biologically aging only 0.7 years and again, while that places me currently between #15 and #16 on the Rejuvenation Olympics leaderboard, it’s not as good as I expected.

So here’s what I plan to do:

These habits will help reinforce a slower pace of aging by optimizing mitochondrial function, inflammation control, and repair signaling.

My OMICm Age results quantify me as 5.2 years younger biologically. That’s within the top 30%, which. is not too impressive. Two markers pulled my score down:

• Versican Core Protein → tissue remodeling, inflammation
• 1‑Stearoyl‑2‑adrenoyl‑GPC → membrane health & neuroprotection

Here’s what I’ll do to improve OMICm further:

Obviously, to dry fast I had to cut the supplements. I already take most of these. I rarely drink and avoid processed foods and oils. I did stop taking omega-3s months ago due to concerns over lipid peroxidation.

A quality, bioavailable fish roe with natural antioxidants remining intact should fix this.

Lastly, My SYMPHONYAge places me biologically younger than 76.4% of peers. Most of my organ systems are already performing well with my hormone and metabolic systems being the priorities to fix.

Again, hard to know the impact of the dry fast here. I’d bet it was significant.

To improve SYMPHONYAge and address my weaker organ systems:

These steps directly address the organ systems dragging down my SYMPHONYAge by balancing hormones, stabilizing glucose, and calming immune reactivity.

Optimize my gut, optimize my BioAge.

TruAge Cost & Accessibility

You can either purchase 1 TruAge test for $499, or subscribe to quarterly tests for $250 per test.

A one-time purchase is a great start to know your biological age and the state of your age biomarkers. You can then make adjustments in your health protocols so they’re tailored to improving your scores.

But you get the most benefit when you test every 3-4 months. This frequency is ideal for closely monitoring the impact of your lifestyle changes on your biological aging rate.

Then, adjust accordingly so that your health routines are always on point, based on objective biological data.

You won’t find this test on Amazon or through most other retailers. TruDiagnostic does have a wholesale program available to health practitioners.

If you plan to retest, the subscription option really makes sense as it makes 4 tests the same price as 2. You’ll receive 4 tests delivered quarterly and pay only $998 for the 4 tests instead of $1,996.

TruAge pricing options

Choose from the following purchase options as per your needs:

• One-time purchase price: $499
• Subscription price: $998 for 4 tests (delivered quarterly)

Who Is TruAge For?

TruAge is one part of the future of precision medicine, providing a functional snapshot of our body’s inner-workings that goes well beyond a traditional blood panel.

Use cases for biological age testing

TruAge supports many health and lifestyle goals, from basic monitoring to targeted optimization:

• Anyone wanting a clear measure of how their body is aging
• People seeking to track health habits’ real impact on biological age
• Biohackers and longevity enthusiasts optimizing for healthspan and performance
• Individuals wanting actionable insights for organ-specific aging
• Those recovering from illness or making major lifestyle changes
• Fitness enthusiasts monitoring training or recovery progress
• People managing chronic conditions who want to assess intervention effects over time
• Health professionals seeking advanced, granular data for clients

When to delay taking a biological age test

It’s best to delay epigenetic methylation testing in the following scenarios:

• Acute illness (wait 4-6 weeks post-recovery)
• Recent major interventions (like my post-fasting timing)
• Pregnancy (wait ≥6 months postpartum)
• Major stress (wait ≥2 weeks post-traumatic events)
• Fasting/caloric restriction (wait 2+ weeks)
• Surgery (wait at least 2 weeks)

These factors may cause temporary methylation changes and skew your results.

Plan testing to reflect longer-term habits, not short-term fluctuations.

Also, testing too frequently without lifestyle changes can lead to confusing results and unnecessary expense. Even without significant lifestyle changes, statistically insignificant variations occur between (high quality) tests.

Other brands can vary dramatically.

Variations occur for all kinds of reasons, including measurement error, slight day-to-day physiological differences, or the inherent variability of biological systems.

If you’re testing often (like once a month or less), you might interpret these tiny, random ups and downs as meaningful trends when they are not.

For the best results, use TruAge when your health is stable and representative of your usual state.

TruDiagnostic TruAge vs. Competitors

TruAge stands above other epigenetic age testing providers because of the following:

• Largest methylation database (>130,000 samples)
• University partnerships (Harvard, Yale, Duke)
• 3rd-generation clocks
• Most comprehensive analysis (850,000+ CpG sites)
• Highest reproducibility (>99%)
• Most biomarkers (180+ total)
• Peer-reviewed publications in top journals
• Clinical trial validation across multiple studies
• Multi-institutional collaboration

If you’d like to know how TruAge stacks up against other biological aging tests, then head on over to my review & comparison of the best epigenetic age tests.

Common TruDiagnostic TruAge Questions & Answers

I’ve collected some common questions about TruAge and answered them for you.

TruAge Review Final Verdict: Is This Test Worth It?

Diagnostic technologies are advancing rapidly, and among those innovations sits TruDiagnostic’s TruAge test.

Not only does it report how each of your biological systems are aging and your overall pace of aging, but the reports also contain actionable recommendations to address imbalances.

I’ve (re) discovered that my focus areas are:

• Gut health
• Inflammation
• Blood sugar management

These were likely influenced by the dry fast just prior to taking the test. Judging by my other markers, when I fix my gut health, the other markers should normalize too.

Most of the insights are not revelations. Core pillars of health like nutrition, movement, recovery, etc are still the highest impact tweaks to bring down biological age.

But these tests are only as good as the data they’re trained on (algorithm). They must be reproducible and accurate.

Few other companies check those boxes.

For biohackers, self quantifiers, longevity enthusiasts, and other nerds—these tests are an exciting breakthrough.

What about you? Have you tested your biological age? Share your experience in the comments below.

TruDiagnostic TruAge

TruDiagnostic TruAge is a comprehensive epigenetic age testing kit that delivers full transparency, industry-leading accuracy, excellent actionable reports, and reasonable pricing.

Product Brand: TruDiagnostic

Editor's Rating:
4.57

Pros

• Comprehensive and actionable reporting
• Fast turnaround
• Scientifically grounded & developed in collaboration with Harvard, Yale, Duke, & other institutions
• Privacy & data control: CLIA-certified & HIPAA-compliant lab
• Global availability
• Official test of Bryan Johnson's Rejuvenation Olympics leaderboard
• Industry-leading accuracy (open source algorithm) and reproducibility

Cons

• Interpreting the detailed reports can be confusing without expert guidance
• Relative novelty and limited third-party reviews